126 THE PLUM CURCULIO. 
ber 28." He cites in detail rearing experiments to support his con- 
clusion, and cites Riley's note in corroboration. 
Riley * fully corroborates the conclusions of Dr. Trimble by rearing 
the curculio in a large cage over a tree, and states emphatically that 
the curculio is single-brooded, but further goes on to say: 
But as there seem to be exceptions to all rules, so there are to this; yet the exceptions 
are only just about sufficient to prove the rule, for as far south as St. Louis not more 
than 1 per cent of the beetles lay any eggs at all until they have lived through one 
winter; or, in other words, where one female will pair and deposit a few eggs the 
same summer she was bred, ninety-nine will live on for nearly 10 months and not 
deposit till the following spring. In more northern latitudes I doubt if any exception 
to the rule will be found. 
During the present study of the curculio but little information on 
the tendency of the insect to produce a second brood under field 
conditions has been secured. Late records of larvae in fruit could 
readily be accounted for as from eggs deposited by the longest-lived 
individuals of the overwintering beetles. 
During 1905, however, at Washington, D. C, a second brood of 
larvae was obtained, though no individuals reached the adult condi- 
tion. Infested peaches were received May 1 from Fort Valley, Ga., 
and confined over moist soil in a large covered glass jar kept in the 
insect ary, where temperature conditions were abnormally high. By 
June 8 many adults were emerging from the soil, and on June 12 
several apples were added. On July 13 eggs were found in four 
apples, and subsequently fertile eggs were laid on the 17th, 20th, 21st, 
22d, and 24th of July, and by August 2 several larvae had developed 
to full size,* some remaining in the fruit and others entering the soil. 
Several larvae were separated for particular observation, but all of 
these died without transforming to the pupal stage, and no adults 
were secured from larvae "entering soil in the breeding jar. 
During the summer of 1910 a second generation was again reared 
under laboratory conditions, at Barnesville, Ga., this time a large 
number of individuals being reared to the adult stage. Adults of the 
first generation were reared out of doors from infested peaches gath- 
ered in an orchard, the beetles beginning to emerge June 6. On 
emerging, the beetles were put in large muslin-covered battery jars, 
100 to 175 beetles to each jar, and kept in the laboratory. They 
were fed on peach foliage and fruit, but were often neglected, allowing 
the jars to become very humid and sometimes moldy. On July 11 
several eggs were found in peaches taken from these jars. The 
beetles, 480 in number, were then supplied with ripe Elberta peaches 
from which all curculio eggs had been removed. On examining this 
fruit two days later 113 eggs were found. Eggs were subsequently 
obtained in abundance, a typical record of eggs laid in fruit left in 
the jars overnight being shown in Table LXXII. 
1 Third Missouri Rept., p. 11. 
