BIOLOGIC NOTES ON SPECIES OF DIABROTICA. 79 
From the above record the stages are as follows : 
Days. 
Egg stage 9 
Larval stage 26 
Pupal stage 11 
Total 46 
The mature larva?, remained quiescent in the cells for 6 days before 
pupating, and the beetles were in the cells from 2 to 3 days before 
issuing. The adults begin to feed as soon as they leave the cells, 
although they are still soft and light colored for from 3 to 5 days. 
No pupa? were found in nature, but in the rearing jars the larvae, 
when mature, burrowed into the soil from § inch to 2 or 3 inches and 
formed cells by wriggling about. The larva? and pupa? were very 
tender and were easily killed if handled at all roughly or if the soil 
in the cages became dry. A moderately moist condition appeared to 
be most favorable. 
Three females, which were placed in confinement during the first 
days of March, deposited respectively during one day as follows; 23, 
45, and 48 eggs. The incubation period of these eggs varied from 9 
to 1 1 days. One of these lots was carried through with the following 
results : 
Days. 
Egg stage 9 
Larval stage 25 
Pupal stage 11 
Total 45 
On April 21, 8 adults which issued in the rearing jars between 
April 18 and April 21 were put together into one cage, with foliage of 
Verbesina, to breed. 
Record of 8 adults of Diabrotica balteata which had issued in rearing jars April 18 to 21 9 
1909, and confined, April 21, in one cage with foliage of Verbesina, to breed. 
1909. 
Mated. 
Eggs de- 
posited. 
Died. 
Escaped. 
April 24.... 
April 28 
April 30.... 
May 14 
May 18.... 
Total . . 


2 







1 
1 
(O) 
1 
2 
1 
2 

5 
1 
o Taken out; see table following. 
None of the beetles deposited any eggs, nor were they observed 
mating. 
