448 
POPULAR SCIENCE REVIEW. 
Two hours later the granular sphere had melted in the isthmus, leaving 
behind what appeared to be a large oil globule and half-a-dozen smaller 
ones. The isthmus in the former time measured ~ mm., at the later time 
— mm. Shortly afterwards, the isthmus elongated and contracted to ^mm. 
on the left, while the right half, retaining the oil globules, remained as 
thick as before. At the same time the animals became flattened at the 
opposite poles. The latter subsequently became depressed, so that the 
animals assumed a reniform outline. The isthmus, now more rapidly 
narrowed and elongated, became a mere thread, and finally separated about 
one hour from the last two hours indicated. The oil globules were retained 
in the right-hand individual, which, with the remaining projection of the 
isthmus, appeared broadly coniform in outline. In the left-hand individual 
all remains of the isthmus at once disappeared, and the animal appeared re- 
niform in outline, but now contracting on the same .side it assumed the 
biscuit form. The constriction rapidly increased, and in thirty minutes 
from the time of separation from the right-hand individual it divided into 
two separate animals presenting the ordinary appearance of Actinophrys Sol. 
Thus this second division took place in an opposite direction from the first. 
The right-hand individual, retaining the oil globules apparently un- 
changed, more slowly assumed the reniform outline, and then became con- 
stricted all around. The constriction elongated to an isthmus, in the centre 
of which were the oil globules. Three hours after the separation of the 
right-hand animal, the isthmus was narrowed to about half the diameter 
of the two new individuals which were about to be formed. At this 
moment other engagements obliged me to leave the examination of the 
animals. Six hours after, in the animalcule cage, I observed only half-a- 
dozen individuals of the Actinophrys Sol. 
