On the Disappearance of the Anthrax Bacillus after Death. 269 
ear-blood when examined on the 10th showed great numbers of anthrax bacilli 
unmixed with other bacteria. The carcass was left unopened for three weeks, 
and the spleen was then removed and left exposed to the air till May 9. 
Result. — The rabbit remained unaffected. 
Experiment V. 
May 9. — The lamb’s spleen referred to in the preceding experiment was 
cut into small pieces and pounded up with about half a pint of water. The 
liquid thus obtained was poured over the throat of a sheep. 
Result. — Negative. 
Experiment VI. 
May 9. — Repeated Experiment III. with spleen-pulp from the cow 
which died of anthrax on March 30. The spleen was now very putrid, and 
no anthrax bacilli could be detected in it by microscopic examination. 
Result. — The rabbit remained unaffected. 
Experiment VII. 
May 9. — Took a portion of the cow’s spleen (weighing about 4 oz.) re- 
ferred to in the preceding experiment, cut it up into small pieces, and 
pounded it with about half a pint of water. The liquid thus obtained was 
poured over the throat of a sheep. 
Result. — Negative. 
Experiment VIII. 
May 9. — Inoculated a rabbit by scarifying its ear and rubbing in pulp 
from the putrid spleen of a sheep dead of anthrax on April 2G. The ear- 
blood examined on April 27 had shown enormous numbers of anthrax bacilli. 
The spleen was left in the body for a week after death, and after its removal 
it stood exposed to the air. 
Result. — Negative. 
Experiment IX. 
May 9. — The putrid spleen referred to in the preceding experiment was 
minced and pounded with half a pint of water, and the mixture, with the 
exception of the coarser particles, was poured over the throat of a sheep. 
Result. — Negative. 
Experiment X. 
May 9. — Inoculated a rabbit by scarifying its ear and rubbing in spleen- 
pulp from a sheep dead of anthrax on April 27. The ear-blood examined on 
the day of death showed anthrax bacilli unmixed with any other organisms. 
The body was left unopened for about a week and the spleen after its removal 
stood exposed to the air. On the date of inoculation no anthrax bacilli could 
be detected in the spleen-pulp by microscopic examination. 
Result. — Negative. 
Experiment XI. 
May 9.— The spleen of the sheep referred to in the preceding experiment 
was minced and pounded with half a pint of water, and the mixture, with 
the exception of the coarser particles, was poured over the throat of a 
sheep. 
Resul t. — Negative . 
J. McFadyean. 
Rov3i 1 Veterinary College. 
VOL. V. T. S. 18 
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