Louping-ill in Sheep. 
551 
Cranial pia mater slightly congested, and excess of cerebro-spinal fluid. 
No bacteria were discoverable by microscopic examination in the spleen-pulp 
or cerebro-spinal fluid. Blood and pericardial fluid from this lamb were 
used for inoculation (Experiment 6). 
Inoculation Experiments with Natural Products. 
Experiment I. — Two half-bred lambs, about 1 month old, were inocu- 
lated with blood and spinal cord of a lamb said to have died from louping- 
ill (Case IV). The inoculation was performed as follows. About 2 c.c. of 
blood from the heart were mixed with an equal volume of sterile bouillon, 
and by tbe aid of a glass rod a piece of spinal cord was mashed up in the 
mixture. The liquid was then divided between the two experimental lambs, 
the seat of injection being the inside of the thigh. 1 
Result. — Negative. 
Experiment II. — A lamb about 4 months old was inoculated with 
about 1 c.c. of cerebro-spinal fluid from a yearling ewe hilled while suffering 
from louping-ill (Case IX.). The fluid had been preserved for 8 days in a 
sealed glass tube. The seat of inoculation was the inside of the thigh. 
Result. — Negative. 
Experiment III. — About ten drops of cerebro-spinal fluid from a ewe 
killed while suffering from louping-ill (Case VIII.) were injected under the 
skin of the thigh of a four mouths old lamb. The fluid had been kept for 
9 days in a sealed tube. 
Res ult. — Negative . 
Experiment IV. — About 20 drops of mixed pericardial and cerebro- 
spinal fluid from a lamb killed while suffering from louping-ill (Case XIV.) 
were injected under the thigh of a four months old lamb. The fluid had 
been preserved for 4 days in a sealed tube, 
Result. — Negative. 
Experiment V. — About 15 drops of pericardial fluid from a lamb dead 
from louping-ill (Case X.) were injected under the skin of the tbigh of a 
four months old lamb. The fluid had been preserved for 5 days in a sealed 
tube. 
Result. — Negative. 
Experiment VI. — About 20 drops of mixed blood and pericardial fluid 
from a lamb dead of louping-ill (Case XV.) were injected under the skin of 
the thigh of a four months old lamb. The blood and pericardial fluid had 
been preserved in a sealed tube for 17 days. 
Result. — Negative. 
Experiment VII. — About 15 drops of heart blood from a lamb dead 
of louping-ill (Case XI.) were injected under the thigh of a lamb about 
four months old. The blood had been preserved in a sealed tube for 19 days. 
Result. — A slight cord-like induration appeared at the seat of inoculation 
on the second day ; it persisted for a few days, and then declined without 
suppuration. The general health of the lamb appeared undisturbed. 
Experiment VIII. — This experiment exactly repeated the preceding 
one, save that the blood had been taken from the heart of Case XIV. and 
preserved in a sealed tube for 18 days. 
Result. — Negative. 
'In all these experiments the usual precautions against accidental con- 
tamination were taken. Fluids were aspirated into tubes previously sterilised 
by heat, and the hypodermic syringes used for injecting had been sterilised in 
the same way. 
o o 2 
