■12 
( Embryo formed in 5 days 
15. Haddock.. 
fresh at 8 p.m 
} and developed successfully. 
( A number unfertilized. 
10. Haddock.. 
at 10 a.m., 2 hours 
17. „ .. 
at 12 noon, 4 hours 
Failed to fertilize. 
18. „ .. 
at 2 p.m., 6 hours 
A number were fertilized. 
(using some fresh milt) 
19. „ .. 
at 4 p.m., 8 hours 
Failed to fertilize. 
20. „ .. 
at 6 - 20 p m., 8 hours 
>1 
The fish 
were kept in a basket and repeatedly stripped as above, 
the female did not appear to be very ripe. 
dOth March. 
21. Haddock . 
. 1 hour ... 1 hour ... 
Fertilized and developed 
successfully. 
21a. „ .. 
• 11 ••• »> ••• 
11 11 
22. Flounder 
1£ hour ... 1£ hour ... 
11 11 
23. Haddock.. 
£ hour ... | hour 
Failed to fertilize. 
evidently not ripe 
31sf March. 
24. Plaice .. 
“living” ... “living” ... 
Many successfully hatched 
Both got on Fish Quay and 
out, but a large number 
fertilized “ dry,” got into 
though fertilized did not 
boxes 4 hours afterwards. 
hatch out. 
25. Flounder 
“living” ... “living” ... 
11 U 
I examined at 3-30 pan. milt of plaice got at Shields from living 
male at 9 30 a.m., and found it quite dead. The milt appears from 
this and other examinations to be much less resistent than the ova. 
The foregoing experiments prove that the ordinary methods of 
fishing and preserving fish on trawlers are rapidly fatal to the 
reproductive elements. They tend to show, moreover, that even 
when the fish are not preserved in ice, the milt and the ova do not 
long survive the death of the fish. The milt very soon dies after 
the capture of the fish, but occasionally a specimen is got which 
retains vitality for some time. This agrees with observations 
communicated to me by Mr. Cunningham and by Mr. Holt. With- 
out laying any degree of stress on the more purely biological aspect 
of the experiments, some of the results almost hint that fertilization 
may be qualitative. 
