DR. EVANS ON THE POISONED SPINES OF THE WEEVER FISH. 367 
containing i C. C. of 5 % washed human corpuscles, were 
numbered o, 1, 2, 3, 4 and 5. 0 was the control. 
1. contained 1 drop 50 % Weever poison. 
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5, ,, 2 drops + 1 C. C. normal serum. 
0, underwent no change after two hours at 37 0 C. 
1, showed medium haemolysis with dark red sediment, 
2, showed well marked haemolysis with dark pink sediment. 
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4, showed very marked haemolysis, almost complete ; sedi- 
ment being merely faint pink. 
=S, showed complete haemolysis. 
There seems no doubt that trachinus venom can dissolve 
human corpuscles without serum, and that normal unheated 
serum does not delay but rather increases the solution. 
Experiments were made to endeavour to activate venom 
insufficient to dissolve the human corpuscles by means of 
Sheep serum and Ox serum. A venom of which one drop did 
not dissolve washed human corpuscles, was mixed with 
.3 C. C. sheep serum, and a control was made with sheep 
serum only. The result was that with one drop Weever 
poison -1- .3 C.C. sheep serum there was marked haemolysis ; 
the control showed agglutination but very faint haemolysis. 
A similar experiment was made with ox serum .1 C. C. of 
which was used for activation. There was complete haemolysis 
with Ox serum + one drop Weever poison, and slight haemolysis 
in ox serum and blood alone. In both the above experiments 
there were two other controls, with the corpuscles and Weever 
poison and no haemolysis occurred. 
I have made experiments with the idea of activating the 
venom towards human blood by means of lecithin, and have 
succeeded on two occasions ; but as other experiments have 
not given uniform results, I am continuing this investigation 
with lecithin recommended by Preston Kyes. 
Horses’ blood gives the haemolytic reaction with washed 
corpuscles alone. 
