542 Proceedings of the Royal Society of Edinburgh. [Sess. 
The cerebro-spinal fluid is more or less increased in amount and contains 
numerous micrococci, free and also included in cells. 
On section there are numerous small haemorrhages scattered throughout 
the brain substance, whilst the velum interpositum is so congested as to 
resemble a clot of blood. Elsewhere the brain appears unaffected to the 
naked eye. Agar tubes inoculated with brain substances from any portion 
of cerebrum or cerebellum or from cerebro-spinal fluid, either cerebral or 
spinal, yield a copious and pure growth of M. melitensis within thirty -six 
hours. 
Thoracic Cavity . — Slight enlargement of anterior mediastinal and of 
bronchial glands. Small quantity of clear serous effusion in the pleural 
cavity. Cultivations from this fluid remain sterile. Few haemorrhages on 
the surface of lungs. Pericardium distended with clear serous fluid, also 
sterile. Agar plate cultivations prepared with blood removed from right 
side of heart yield on an average some 35 colonies per cubic millimetre. 
The agglutination titre of the serum = 1 : 600. 
Peritoneal Cavity . — Excess of clear, blood-stained fluid in peritoneal 
cavity. Gall-bladder distended with clear bile. Liver, spleen, kidneys, 
dark and engorged with blood — spleen being distinctly enlarged. 
Omentum injected : a few large mesenteric glands noted. Bladder 
distended with turbid urine. 
Cultivations from liver, gall-bladder, and spleen give good growth of 
M. melitensis within forty-eight hours. Kidney pulp yields only a few 
scattered colonies of M. melitensis. 
Cultivations prepared from the centrifugalised deposit of the few cubic 
centimetres of urine contained in the bladder remain sterile. 
Cultivations from the bone marrow from practically all the long bones 
yield a more luxuriant growth of M. melitensis than from other organs, 
with perhaps the exception of the spleen and brain. 
The accompanying chart (No. 1), showing the hourly temperature, 
weight, and sedimentation value of the serum in a more acute case still, 
where death occurs in twenty-seven hours, exhibits clearly the characteristic 
features of the infection. 
2. Chronic Infection . — After intracerebral inoculation with a very 
minute dose of a highly virulent culture or a fair-sized dose of a less 
virulent one, the infection pursues an extremely chronic course, and beyond 
progressive emaciation and profound anaemia presents no very marked or 
characteristic symptoms. The early symptoms resemble those of the more 
acute infection above described, but are much less severe in character. For 
instance, the incubation period is usually prolonged to two or three days, 
