74 
C. H. MARTIN AND MURIEL ROBERTSON. 
possibly play an important part in the life-cycle of the 
Trichomonas. These forms possibly represent some con- 
jugation process, but at present we can offer no decisive 
evidence upon this point. It is, of course, possible that 
they are degenerate dividing forms. They may be tersely 
characterised as forms with a double nuclear apparatus and a 
double chromatic line, but in which the flagellar apparatus is 
degenerate, and finally becomes entirely lost. In the later 
stages of this process a number of forms are met with in 
which the chromatic lines become twisted into irregular 
circles. 
The nuclei of the specimen shown in PI. 13, fig. 48, would 
appear to be undergoing division, possibly preparatory to 
conjugation, and it is interesting to note that forms of this 
type with nuclei containing only four chromatic masses 
have been found, e.g. fig. 49. On the other hand, it is 
possible that the division of such a form as this would give 
rise to the resting forms we have previously described. 
Against this view, however, it may be noted that on one 
occasion we had an opportunity of keeping a living form of 
Trichomonas Eberthi, with two membranes and no free 
flagella, under observation for sis hours, during which time 
there was not only no sign of division, but the animals 
became more rounded and lost one membrane. It seems 
very possible that it is to such forms as these that Schaudinn 
refers on p. 550 in his paper, “ Untersuchungen uber die 
Fortpflanzung einiger Rhizopoden ” (1903). 
“Die in fast jedem Darm vorkommende Trichomonas 
intestinalis verliert namlich vor der Kopulation ihre Geisseln 
und ist dann von einer echten Amobe nicht leicht zu unter- 
scheiden, weil sie auch mit stumpf-lobosen Pseudopodien 
umherkriecht. ,, 
7. Trichomastix Gallinarum. 
The body in the fully developed active form of Tricho- 
mastix gallinarum is roughly pear-shaped, measuring 
about 5 fx long by 3 fi . 
