FURTHER OBSERVATIONS ON C^CAL PARASITES OF FOWLS. 77 
membrane and the karyosome, characteristic of the recently 
divided nucleus figured and the state shown in fig. 59, i 11 
which the chromatin is assuming the irregular distribution 
through an oval nucleus characteristic of the later stages of 
division and the normal active form. 
In the later stages the line between the blepharoplast has 
disappeared, and the fourth free flagellum has grown out in 
the case of each individual. The process of division is in 
these cases complete except for the severance of the cytoplasm. 
From the occurrence, however, of free individuals with only 
three free flagella, it seems probable that in some cases the 
fourth flagellum is only added after the division of the 
cytoplasm has been effected. 
The aflagellate resting forms of Trichomastix galli- 
narum are fairly commonly met with, lying usually in groups 
of three or four, in preparations from the cascal wall. 
In the typical resting forms, such as are figured on PL 14, 
fig. 64, the body of the animal is quite rounded. There is no 
trace of flagella, but there is a well-marked blepharoplast. 
The nucleus has the characteristic appearance already 
described for the active form, but there is a tendency for 
the chromatin granules either to become massed in a double 
row or to fuse, forming a darkly staining band round the 
periphery. An axostyle may be present or absent. 
It is probable that the grouping of these resting forms 
mentioned above is due to antecedent division. 
In fig. 65, PL 14, a group of three distinct individuals is 
drawn, of which A is just dividing, whereas b and c are pro- 
bably the result of a recent division. 
If this hypothesis of division be accepted, it is rather 
interesting to note that in each of the cases figured (PL 14, 
fig. 65), the division of a presumably non-flagellate form has 
resulted in the production of one individual in which new 
flagella are growing out, whilst the other individual still 
remains in the resting stage. A later stage in the outgrowth 
of the flagella in a form probably derived from the division of 
a resting form is seen in fig. 66, PL 14. 
