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W. GLEN LISTON AND C. H. MARTIN. 
pathogenic, which he named Entam oeba histolytica. A 
number of other workers using his methods have discovered 
still other species, viz. E. tetragena, E. minuta, etc. 
More recently Musgrave and Clegg (1) in the Philippine 
Islands, and Noc (2) in French Cochin China, have stated 
that they have isolated by culture from liver-abscesses and 
dysenteric stools a somewhat polymorphic amoeba, which 
they regard as the causative agent of liver-abscess and 
dysentery in their respective countries. They have also 
found the same species in a number of other situations — in 
drinking water, on vegetables, and in the intestines of 
healthy men and animals. In fact, they seem to think that 
all amoebae which can be cultivated on their agar medium, and 
which form cysts varying in diameter between 7 and 16/m, 
belong to one species, 1 a species which can at one time pass 
a harmless existence outside the body, at another time, when 
conditions are favourable, invade the tissues, and give rise to 
the grave lesions associated with dysentery. 
The present paper has been written to show that at least 
two very distinct species of amoebse haYe been found in 
cultures obtained after the manner of Musgrave and Clegg. 
I am, however, not yet in a position to state whether both or 
either of these species are really the causative agents of 
dysentery and liver-abscess. I am indebted to Captain Wells, 
of the Indian Medical Service, who has for some time been 
1 While Musgrave and Clegg in their monograph on “ Amoebas ” 
{Bureau of Govt. Laboratories, No. 18, p. 77), state that “The cultiva- 
tion of pure species of aincebas has offered strong evidence of the 
plurality of the species of these protozoa, and this plurality apparently 
extends to those which produce infection in man.” in a later publica- 
tion Woolley and Musgrave, in a paper published in the same series, 
No. 32, June, 1905, write : “ It may be well to. state at the outset that 
we can see no valid reason for departing from the nomenclature of 
Losch. He described a pathogenic amoeba and called it Amoeba coli. 
Why this term should be applied to a suppositious n on-pathogenic 
organism it is difficult to say. We shall, in referring to the cause of 
intestinal amoebiasis, use the name introduced by Losch.” The measure- 
ments of the cysts are those given by Noc. 
