STUDY OF PATHOGENIC AMCEB2E FROM BOMBAY. Ill 
and figured by Noc (2), who describes the buds he observed 
as very small, each bud containing a single minute fragment 
of chromatin. So numerous were the buds within the body 
of the parent amoeba that he says the term “ merozoite 99 
could be aptly applied to the bred. In my experience 
great difficulty was found in differentiating between the 
bodies of bacteria ingested by an amoeba and lying within 
its cytoplasm and small fragments of chromatin or chromidia. 
It must be remembered than in cultures the amoebae are 
always present with vast numbers of bacteria. Although 
a number of stains were used none was found which satis- 
factorily distinguished bacteria from chromidia. It was 
therefore difficult to trace the origin of the buds. The buds 
became recognisable within the cytoplasm of the parent 
amoeba, when a larger mass of chromatic material was 
assembled than could be reasonably explained on the sup- 
position that it was formed from ingested bacteria. Around 
these collections of chromatic material the cytoplasm of the 
amoeba becomes differentiated from the rest of the cytoplasm 
of the body. As this differentiation becomes more and more 
marked the enclosed chromatic material more and more 
closely resembled the structure of a typical nucleus, till 
finally, within the body of the amoeba, a completely differen- 
tiated amoebula is developed. At this stage the amcebula 
escapes from the parent amoeba, and can then live a free 
existence, grow and develop into a full-sized adult amoeba. 
Division of this large amoeba from the liver-abscess 
culture into two individuals was also observed in the living 
condition. On one occasion a single amoeba was watched con- 
tinuously for two hours and fifty-four minutes, and a detailed 
record was kept of the various events that occurred during 
this period. The culture in which this amoeba was observed 
had been planted five and three-quarter hours before the 
observations commenced. A number of amoebae had already 
escaped from their cysts while still others had not yet 
emerged from their cysts. The amoeba which was selected 
for observation was a very active one. In progressing over 
