STUDY OF PATHOGENIC AM(EBiE FROM BOMBAY. 113 
the protoplasm became more and more granular. The 
nucleus, which up to this point had been clearly visible, could 
now no longer be distinctly defined. No movement of any 
sort was noticed for a few minutes. Some ten minutes 
after the amoeba had engulfed the last cocci, and a few 
minutes after all movement had ceased, the amoeba slowly 
changed from a rounded to a more oval form, becoming 
oblong, then elongated. Two small indentures next appeared 
in the protoplasm on either side, about the middle of the 
body. These indentures deepened, till, meeting, two amoebae 
were formed. Division was complete in three minutes after 
the first appearance of the indentures mentioned above, and 
about thirteen minutes after the last cocci had been taken up 
as food. No trace of a nucleus could be made out in either 
of the two new amoebae immediately after division. The 
newly formed amoebae soon moved away from one another, 
and in three minutes one of them had approached and 
engulfed a group of nine cocci. A nucleus now gradually 
appeared in each amoeba, but not till five minutes after 
division had been completed could it be clearly defined, 
meanwhile, as has been remarked above, the amoebae moved 
about and even fed. Each amoeba by this time had a 
contractile vacuole which rhythmically contracted about once 
every two minutes. The young amoebae were watched for 
nearly an hour longer; no fresh points of interest, however, 
were noticed. The observations were abruptly terminated 
by an accidental knock to the Petri dish, which caused the 
amoebae under observation to be moved out of the field of 
vision, and made it impossible to recognise with certainty 
those which had been watched from other amoebae in the 
culture. I have not been able successfully to follow in a 
series of stained specimens the various changes described 
above seen in the living amoeba?. (Since writing this, with 
the assistance of Mr. C. H. Martin, divisional forms have 
been studied in stained specimens. The division is indirect 
or by mitosis.) 
While thus the large amoeba from the liver-abscess culture 
