114 
W. GLEN LISTON AND C. H. MARTIN. 
showed two methods of multiplication, the small amoeba, 
cultivated from the small cysts of the same culture, was 
never seen to give off buds. Actual division in the living 
state was not observed in the case of this amoeba, but in 
stained preparations dividing forms were fairly easily found. 
Division in this case is direct or amitotic. Three such forms 
have been sketched from a single slide, while in other slides 
other stages of division have been observed. 
(b) The Behaviour of the Amceba in Saline Solution. 
One of the most striking differences between the two 
species of amoebae found in the liver-abscess culture was 
brought into evidence when dilute saline solution 5 per cent, 
was added to an actively developing culture, time being 
allowed for the amoebae to recover from the shock produced 
by the addition of the liquid. To demonstrate this difference 
between the two species of amoebae the best results were 
obtained by placing a drop of the mixture of the amoebae 
and saline solution on a slide and allowing it to remain for a 
few minutes in a warm moist chamber before fixing in 
Flemming’s solution or sublimate-acetic mixture. The drop 
containing the amoebae must not be covered with a cover- 
glass, but should be spread out in a thin layer and left freely 
exposed in a moist chamber. Adopting this method of pre- 
paring and fixing specimens, it was found that the large 
amoeba always assumed a more or less rounded form, with 
lobose and numerous fine needle-like pseudopodia projecting 
from the surface. The small amoeba on the contrary almost 
always assumed an elongated worm-like or gregarine shape, 
throwing out one or two long lobose pseudopodia either 
directly in front or often from the side so that L-shapes and 
Y-shapes, as well as long I -forms, were developed. 
That Noc was dealing with both these types of amoebae is 
evident, not only from the measurements of the cysts he 
worked with, but also from the drawings on his plate X. The 
drawings show both forms, the majority representing the 
