STUDY OF PATHOGENIC AMCER® FROM BOMBAY. 
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large amoeba type, while his fig. 20 is a typical specimen of 
the small amoeba described above. 
(c) Minor Points of Difference. 
Other less important differences were noted between the 
two types of amoeba found in the liver-abscess culture. Thus, 
for example, the nucleus of the large amoeba in the living 
state was more clearly seen than that ot' the small amoeba. 
The ectoplasm, too, was more clearly differentiated from the 
endoplasm in the case of the large amoeba than in the small 
amoeba. Then, again, a yellow pigment-producing coccus, 
which was a peculiarly favourable organism to grow with the 
large amoeba, appeared to be quite unsuitable for the smaller 
amoeba. 
From what has been said above, it is evident that two very 
distinct species of amoeba were present in this liver- 
abscess culture, and although a somewhat limited arnouut of 
attention has been given to the cultures derived from other 
sources, it is possible that the cultures from the Bombay City 
water aud from the monkey, which contained an amoeba very 
closely resembling the large amoeba from the liver-abscess 
culture, nevertheless differed from it. The small amoeba 
from the liver-abscess culture very closely resembled that 
found in the culture from a dysenteric stool. 
An attempt was made further to differentiate between the 
two species of amoebae found in the culture from liver- 
abscess by carrying out experiments on animals. For the 
purpose of these experiments young kittens were selected. 
The two species of amoebae were isolated in pure mixed 
culture, and, when the amoebae had encysted, an emulsion of 
each species was made in saline solution. Four kittens, 
whose faeces in the first instances had been examined micro- 
scopically and by culture for the presence of amoebae with 
negative results, were experimented upon as follows : Two 
were fed by means of a small stomach-tube, the one with an 
emulsion of large amoeba-cysts, the other with an emulsion 
