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W. GLEN LISTON AND C. H. MARTIN. 
alive, stimulate the amoebae to multiplication, when boiled 
and eaten by the amoebae lack this power. 
Amoebae have been seen to feed upon and digest red blood- 
corpuscles, but in the absence of liviug bacteria failed to 
multiply on agar cultures. 
Lister Institute, 
November, 1910. 
Part II. — Descriptions of Preparations of Amoebae from Major 
Liston’s Cultures. By C. H. Martin. 
In November of last year, Major Liston was kind enough 
to hand over to me some preparations and cultures of 
amoebae, with a request that I should look through them. 
The preparations were made from cultures of five different 
strains of amoebae, under the circumstances which Major 
Liston has described in Part I of this paper. 
Major Liston has given an account of his observations on 
three of these forms, and since the live cultures unfortunately 
dried off in my hands before sub-cultures could be made, it 
only remains for me to add a few notes on the details of 
division and budding from the stained films of the larger type 
of amoeba from liver-abscess. I have also, for the sake of 
completeness, given a short description of the other amoebae, 
in the hope that I may be able at some future date to give the 
results of further work on live cultures. 
The Larger Type of Amceba from Liver-Abscess. 
As will have been seen already from Major Liston's des- 
cription, this is a very well-marked form, both from a 
morphological and a physiological standpoint. In stained 
preparations the full-grown amoeba is characterised by a 
nucleus in which the mass of the chromatin is condensed 
into a large round karyosome (PI. 16, figs. 1-5). In addition 
to the chromatin of the karyosome, there is a cloud of fine 
