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figs. 8 and 9) the chromatin-masses have divided and 
separated slightly, and it is, I think, interesting to note, 
from a comparison of the early and late stages of division- 
figures (PI. 16, fig. 7 and fig. 10), that the separation of the 
chromatin-masses does not appear to be due in any marked 
degree to the shortening of the polar threads of the spindle 
between the centrioles and the chromatin-masses, but rather 
to a growth and elongation of the separation-spindle between 
the two daughter-plates. In the still later stage of division 
shown in PI. 16, fig. 11, the upper nucleus has practically 
assumed the resting condition, and would seem to show that 
the chromatin of the daughter-plate forms the mass of the 
karyosome, in which the centriole is probably included. 
(2) Budding. — The most common form of reproduction 
for the larger type of amoebae from liver-abscess is effected 
by the formation of fairly large buds, as has been described 
already by Major Liston in the account of his observations 
on the live amoebae. There is no evidence on the stained 
films of this form for the formation of the numerous very 
small buds described by Noe (2) for v a similar amoeba 
obtained by him from liver-abscesses in Cochin-China. In 
the majority of the forms studied by us only one bud is 
formed at a time, though there are cases where this number 
may be exceeded (PI. 16, fig. 4 and fig. 10). 
The nucleus of the amoeba takes no direct part in the 
formation of the bud. There is absolutely no evidence, 
either from observations on the live amoebae or from the 
stained films, for any form of nuclear division connected with 
the bud-formation; and, on the other hand, it is quite clear 
from the stained preparations that the cytoplasm of the bud 
is completely cut off from that of the parent at a stage in 
which the bud shows no distinct nucleus. A comparison of 
different stages in the growth of the bud shows that the nucleus 
of the bud arises from chromidia contained in it when it is 
first formed, and derived from the chromidia scattered through 
the cytoplasm of the parent. 
When first recognisable, the bud is seen as a small sphere, 
