STUDY OF PATHOGENIC AMCEB^E FKOM BOMBAY. 
125 
about 3 jjl in diameter, separated from the surrounding cyto- 
plasm of the parent by a clear space (PI. 16, fig. 6 ; PL 17, fig. 
19) ; at this stage the substance of the bud shows no difference 
from that of the parent cytoplasm, except that with TworPs 
stain it stains a deeper red (PI. 17, fig. 19), indicating that 
the chromidia are present in greater quantity, probably as the 
result of growth and increase of the chromatin-substance in 
the bnd itself after it lias been cut off from the parent. The bud, 
imbedded completely, in the maternal cytoplasm, grows until 
it attains a diameter of 9-10 ft or more. The chromidia con- 
tained in the bud condense into irregular strands of chromatin 
(PL 16, figs. 2 and 3; Pl. 17, figs. 17, 18), which gradually 
become arranged into a nucleus of the type ot‘ that possessed 
by the parent (PL 16, fig. 4; PL 17, fig. 19). But by no means 
all the chromidia contained in the bud are used up to form 
its nucleus ; a certain number remain over (PL 16, figs. 3, 4, 6 ; 
Pl. 17, figs. 18-21), so that the fully formed bud contains 
chromidia in its cytoplasm in addition to a nucleus. The bud 
at the time of its escape may show a well-developed nucleus 
with a karyosome (Pl. 16, fig. 4), but more frequently this 
change is completed after the young amoeba is set free (PL 16, 
fig. 6). The young amoebae begin to form buds in their turn 
long before they are full grown (PL 17, fig. 21). 
The question of the cysts is rather a difficult one, and 
needs further work on live cultures. In smears from 
cultures about eight hours old amoebae may be seen stiil 
enclosed in a smooth-walled cyst with the vacuole which 
Major Liston has described as being developed in the 
escaping form (PL 16, fig. 12). On the other hand, empty 
rough-walled cysts are also found. The latter may be due to 
a shrinkage of the cyst after the amoeba has escaped, but it 
must be confessed that they seem rather too large to be 
explained on this hypothesis (PL 16, fig. 13). 
The Smaller Type of Amceba from Liver-Abscess and the 
Dysentery-Amoeba:. 
The small type of amoeba from liver-abscess (PL 18, figs. 
22-24), and the dysentery -amoebae (PL 18, figs. 25-29), seem 
