STUDY OF PATHOGENIC AMCEBiE FROM BOMBAY. 127 
References. 
1. Musgrave, W. E., and Clegg, M. T. — “ Amoebas : tlieir Cultivation 
and iEtiologic Significance,” 4 Manila, Dept, of the Interior, 
Bureau of Government Laboratories, Biol. Lab.,’ No. 18, 85 pp., 
32 figs. (1904). 
2. Noc, F. — “Reclierches sur la dysenterie amibienne en Cochin- 
Chine,” ‘Ann. Inst. Pasteur,’ xxiii, pp. 177-204, pis. x-xiii (1909). 
3. Schaudinn, F. — 44 Unter such ungen fiber die Fortpflanzung einiger 
Rhizopoden,” 4 Arb. k. Gesundheitsamt. Berlin,’ xix, pp. 547-576 
(1903). 
EXPLANATION OF PLATES 16-18, 
Illustrating Major W. Glen Liston and C. H. Martin’s 
IC Contributions to the Study of Pathogenic Amoebae from 
Bombay.” 
[All the figures are drawn with the camera lucida to a magnification 
of 2000 linear, using Zeiss objective apochr. 3 mm. liomog. imm. N.A. 
1*40, compens. ocular 18.] 
PLATE 16. 
Large type of amoeba from liver-abscess. All the preparations are 
stained with Heidenliain’s iron-hsematoxylin, after fixation with 
Schaudinn’s fluid 4>r sublimate-acetic. 
Figs. 1-6. — Stages of endogenous bud-formation. 
Figs. 1 and 2. — Early stages; in fig. 2 irregular chromidial strands 
are seen in the bud. 
Fig. 3. — Later stage ; the chromidial strands are condensing to form 
the nucleus of the bud. 
Fig. 4. — Amoeba showing six endogenous buds, one of which has the 
nucleus completely formed. 
Fig. 5. — Extrusion of a fully-formed bud. 
Fig. 6. — Free bud, with nucleus not quite fully-formed. 
Figs. 7-11. — Karyokinesis and cell-division. 
Fig. 7. — Early stage; the spindle is fully-formed, but the equatorial 
plate is not split. 
Fig. 8. — Splitting of the equatorial plate. 
