ON THE TRYPANOSOME OF THE LITTLE OWL. 
159 
length approached the female gamete, and after some gyra- 
tions, which caused the latter to be jerked about as well as 
the corpuscles in the vicinity, it was seen to be definitely 
attached to the female by one extremity. The male element 
continued to lash its body and jerk the female about, but 
after a time these movements became feebler and the male 
seemed to be contracting. At this moment the female was 
suddenly violently jerked, and after that no further move- 
ments were seen on the part of the parasite. The female 
individual was now examined very carefully but no signs of 
any little body attached to it could be made out; the male 
gamete seemed to have been absorbed. At this period no 
definite nucleus could be distinguished in the female ; one part 
of the body was clearer and free from pronounced granules, 
but no sharply contoured nucleus could be made out. After 
watching the parasite for some time it was noticed that a red 
corpuscle, flowing slowly past it, was deflected slightly from 
its course, and it was seen that there was then a small body 
attached to the parasite at this point ; this little body was 
spherical, of a definite contour, and contained a few granules ; 
it appeared like a minute cell or nucleus. Shortly after this 
minute body had been found and when our attention was 
again turned on the parasite proper, it was observed that its 
nucleus was now quite distinct. It could be seen as a slightly 
oval clear space, with sharp and definite contour, and had 
near one end a dull spot, quite different in appearance from 
the much darker grains in the cytoplasm. The diameter of 
the nucleus was about double that of the little body attached 
to the female gamete. The parasite was watched for some 
time longer, but the only change observed was that the 
attached body seemed to become rather contracted and 
shrivelled, as if degenerating. No indications of any other 
change or development in the parasite itself could be noticed. 
When seen again in the morning (of the same day) the 
Leucocy tozoon had become hyaline in appearance and 
seemed to be dead ; there was no sign of the little attached 
body. 
