STUDIES IN THE EXPERIMENTAL ANALYSIS OE SEX. 255 
at the Gf. If the solutions of either yellow or pink lipoclirome 
are too strong, a broad diffuse band is formed cutting* off the 
whole of the blue part of the spectrum. These absorption 
bands are the same as those found by Haliburton and other 
observers for the lutein of the hen’s egg-yolk in the case of 
the yellow substance, and of the tetrouerythrin from the 
•blood and eggs of Crustacea in the case of the red. The 
waxy mixture of fatty acids and lipoclirome, in which a 
certain amount of cholesterol may be present, contains a 
mixture of fatty acids, part of which is liquid at room tem- 
perature, while the melting-point of another portion is rather 
above 40° C. The mixture as a whole liquefies between 30° 
and 40° C. 
The blood for the estimations was collected and pooled 
under three categories, non-pig men ted of both sexes, 
pink from males and yellow from females, and the amount 
of ether- soluble substances present was calculated as a per- 
centage on 100 gr. of the original blood. The following table 
gives the results of the experiments. 
Percentage of etlier-soluble substances in the 
blood of Carcinus macnas. 
Non-pigmented. 
Pink. 
Yellow. 
073 per cent. 
•087 
•099 per cent. 
•084 
458 percent. 
103 
035 
•059 
156 
074 
•088 
•203 
034 
•07 
•373 
•086 
•093 
135 
•058 
•095 
•320 
•045 
•103 
143 
•035 
•07 
•062 
Average ’059 per cent. 
Average '086 per cent. 
Average *198 per cent. 
