NUCLEAR DIVISION OF THE LARGE AMCEBA. 
270 
A Note on the Early Stages of Nuclear Division 
of the Large Amoeba from Liver-abscesses. 
By 
C. II. Martin, M.4. 
With 7 Text-figures. 
From some cultures which Major Liston was good enough 
to send me in the course of the summer, I have been able to 
obtain the early stages of the nuclear division of the large 
amoeba from liver-abscesses. 
From a pathological standpoint it may be interesting to 
note that Major Liston has since succeeded in cultivating the 
same amoeba on plates exposed to the air in an Indian jail, 
and that it seems probable that the amoeba cultivated from 
Bombay tap-water is also the same species. 
It does not seem to me that these observations of necessity 
invalidate the possibility that this amoeba is one of the agents 
in the production of liver-abscess, but they certainly point to 
the crying need for further experimental work on this 
subject. 
The first indication of nuclear division is far more clearly 
seen on preparations stained with haemalum than in those 
stained with iron-haematoxylin. 
In these preparations a number of rounded amoeba? are 
met with in which the extra-karyosomic chromatin appears 
to have been absorbed by the karyosome, which is much 
swollen (Text-fig. 1) and in some cases vacuolar. 
The nucleus at a slightly later stage loses its spherical form 
and at the same time the karyosome breaks up into a number 
of lightly staining irregular masses (Text-fig. 2). 
