346 
CRESS WELL SHEARER. 
Autumn. Winter. 
21 
a 
1 
S 
CO 
40 
. 31 
Capsule 
f '0 
CO 
00 
? 
22 
2 
a 
6 
33 
. 32 
33 
1 „ 
2 
33 
23 
a 
3 
a 
8 
33 
. 33 
33 
1 „ 
3 
33 
24 
a 
4 
>} 
10 
33 
34 
33 
1 „ 
2 
33 
25 
2 
3 
33 
35 
33 
1 „ 
2 
33 
26 
j) 
4 
)} 
8 
33 
. 36 
33 
3 „ 
6 
33 
27 
j } 
2 
a 
5 
33 
. 37 
33 
1 * 
3 
33\ 
28 
}> 
1 
}i 
O 
O 
33 
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00 
33 
1 „ 
2 
33 
29 
}} 
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o 
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6 
33 
39 
33 
4 „ 
10 
33 
30 
}> 
1 
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3 
33 
. 40 
33 
2 „ 
5 
33 
Total 
23 
s 
55 
? 
Total 
is a 
43 
? 
Dinophilus is a remarkabty difficult animal to fix well, 
as even the most rapid of penetrating fixatives allows of con- 
siderable shrinkage before it can act. In this respect D. 
toeniatus is even more difficult to handle than D. gyro- 
ciliatus. I am inclined to attribute this to the fact that 
the animals are surrounded by a thin layer of mucus of an 
oily nature that effectively prevents the rapid penetration of 
the fixing fluid. As my interest in Dinophilus lay entirely 
in the direction of obtaining good fixation of the ovary, germ- 
cells, and ova, the fixatives I have used have been judged 
entirely by the results they have given with regard to these 
alone, and for this reason they may have given indifferent 
fixation of the tissues in general. 
The large female eggs are yolky and granular, and on this 
account crumble and break readily in sectioning. I have for 
this reason tried to modify all my fixatives with the object of 
avoiding this as much as possible. This I have done by the 
addition of a small quantity of HN0 3 to my solutions, which 
renders the eggs much less brittle. The fixatives that on the 
whole have given the best results are sublimate-nitric, or 
acetic, and Perenyi’s solution, the sublimate solution consist- 
ing of a saturated solution in either sea-water or distilled 
water, to which 2 per cent, nitric acid has been added. The 
best results were obtained by using the sublimate boiling hot. 
