POLLEN-BODIES OF THE ANGIOSPERMS. 
25 
by culture^ I therefore prepared them independently from 
pollen-besprinkled pistils. In Anthericum liliacjo the whole 
vegetative cell is found in the tubes, together with the 
nucleus of the big cell, which is very much elongated 
and often looks like a very fine thread. It is usually 
this nucleus which goes first, though not without excep- 
tion. Fig. 7 shows such a tube, which also exhibits 
the rather rare instance of branching. On the other hand, 
in Anthericum ramosum I cowld observe no nuclei as soon 
as the tubes w'ere formed. 
It is very much the same with Glohha bracteata^ only that 
in this case one can indicate no special place in which the 
vegetative cell will be formed, owing to the Pollen-body 
being spherical and provided Avith a membrane equally thick 
all over. In its ripe condition the Pollen-body is shaped 
like that of Anthericum (fig. 4). The Pollen-bodies, in a 
solution of 5 per cent, of sugar, produce short tubes; these, 
however, stop too short to allow of conclusions about the 
behaviour of the nuclei. 
Tulipa Gesneriana (figs. 9 — 14) corresponds in most 
particulars with Anthericum. The vegetative cell is enor- 
mously developed (fig. 12 shows two pressed out), and its 
nucleus is often provided Avith several nucleoli. In 
the ripe Pollen-body it immediately strikes one from its 
size and half-moon shaped form. Occasionally I have found 
a division of the vegetative cell in the younger Pollen-bodies 
(fig. 18). In the tubes (1 — 8 per cent, sugar solution; 
eighteen hours) the nucleus of the big cell Avent before, and 
was folloAved by the elongated vegetative cell (fig. 14). 
In Ornithogalum pyramidale the vegetative cell is also 
formed in a corner of the Pollen-body, opposite to the 
opening in the extine. In one instance, Avith osraic acid 
and carmine preparations, the division Avail betAveen 
the tAvo cells clearly appeared double-contoured and was 
highly refractive like the intine, into Avhich it undoubtedly 
passed over (fig. 15). I have no doubt that in th-s case a 
cellulose membrane Avas really formed. 
Its further development corresponds with that Avhich has 
already been described. The vegetative cell becomes finally 
much elongated by stretching, and has pointed, often bent 
in, ends. The greatest part of it is occupied by the almost 
cylindrical nucleus Avhich possesses no nucleolus (figs. 16, 
17). On crushing out the contents of a ripe Pollen-body the 
vegetative cell appears as a hyaline body in the midst. 
Both ends are filled Avith yellowish coloured little bodies ; 
in general it has a strong resemblance to a cell nucleus, for 
