FRED. ELFVING. 
. 24 
glass shaped wall, to which the inline is attached ; it is 
distinguished by its transparent almost homogeneous proto- 
plasm, by its roundish oval nucleus, the nuclear body of 
which, though large, is still smaller than that of the larger 
cell. A partition by means of a cellulose membrane, as in 
the Orchids, seldom takes place, as the result of this division. 
The two cells, are oftener only separated by a layer of cortical 
plasma, which originates from the cell })late.” 
The vegetative cell soon separates itself from the inline 
altogether, and appears as a spherical formation in the inside 
of the Pollen-body (figs. 2 and 3). This cell elongates 
itself considerably in length and becomes spindle shaped 
v,dth pointed ends, which are bent in (figs. 4 — 6) ; its nucleus 
remains almost unchanged. If the Pollen-bodies are care- 
fully crushed in a saccharine solution (5 per cent, strong), 
this nucleus is easily found intact among the contents, which 
are pressed out. When pressed out of rather young Pollen- 
bodies, it generally becomes rounded and assumes a spherical 
shape, although it may have had already in the Pollen- 
body the spindle shape. In ripe Pollen-bodies, on the other 
hand, it is tolerably resistant, and keeps its form. Its 
nucleus appears clearer than the surrounding protoplasm if 
osmic acid is added ; it, however, stands out darker. When 
a strong solution of sugar — the best is one of 20 per cent. — 
is used, or, when a weak agent is a,dded capable of absorbing 
water, the inner plasm structures will be seen to contract and 
to leave behind as a membrane the outer cortical plasm. 
The whole is coloured brown by a chloride of zinc solution. 
While the vegetative cell changes in the manner just 
described, the nucleus of the big cell remains at first unal- 
tered. It afterwards becomes longish, and in doing so often 
bends upon itself. Upon this its nucleolus also vanishes. 
It is very difficult to point out the nucleus in the ripe Pollen- 
* body without using staining materials. The nucleus appears 
then as an irregularly-shaped, often crumpled body, or as 
membranous and shrunk together (fig. 6). 
Antherictm liliago corresponds with the above species, 
except that the large nucleus keeps its round form. In a 
Pollen-body, which lay already on the stigma, but had pro- 
duced no tubes, the nucleus was apparent, when fixed with 
osmic acid and coloured with carmine, as a strangely star- 
shaped body (fig. 8), and gave one the idea that it might 
have performed amoeboid movements while it was being 
fixed. Direct observation on the living Pollen-bodies was 
impossible on account of the thick contents of their cells. 
Pollen-tubes could not be obtained from these two species 
