THE BACILLUS OF LEPROSY. 
95 
Preparation No, 1. — Procured after incision by pressure on 
the tubercle which is tolerably juicy, contains a consider- 
able number of large brown elements and numerous bacteria ; 
these could also be indistinctly seen within the cells w'hich 
are not of a very deep brown, and I think now and then 
that I could distinctly see in these a long stripe in the 
apparently granulated masses. 
No. 2. Like No. 1 
No. 3. Like No. 1 (with water). The cells for the most 
part were swollen, and in these rod-shaped bodies in large 
numbers were distinctly to be seen. The large brown 
elements w'ere not much affected by water, occasionally a 
little swollen, and thus it can be tolerably clearly seen that 
in general a large portion of the apparent granulations are 
oblong and rod-shaped. 
All these preparations were kept in a moist chamber. The 
addition of acetic acid does not help at all. The preparations 
become more opaque by coagulation. 
With acetate of potash all the oscillating rods are killed ; 
they become instantly more highly refractive after it has 
penetrated, shrivel, and lie exactly like corpses all over 
the preparation ; the brown ones shrivel considerably, and 
become exceedingly refractive, shining like >vax. Their 
whole contents become as if kneaded together ; no rods to be 
distinctly seen unless action be just about to commence. The 
rod-like bodies in the cells are to be seen best by teasing 
out a piece of the tubercle in 1 per cent. “ osmic acid.” 
20th. — In many places in all the three preparations arti- 
culated threads of greater or less extent are to be found ; in 
some places a single thread wound a few times round itself, 
in others such a confusion of threads that to follow them w^as 
impossible. 
Of the tubercle which, after obtaining the above-men- 
tioned preparations on March 18th, was laid in 1 per 
cent, osmic acid, a preparation was taken to-day, which 
showed rod-shaped bodies in most of the cells. I took 
a drop of the reduced acid in which the tubercle was 
lying ; no oscillating bodies were to be found ; a smtill 
particle of the tubercle w’as placed into this drop and teased 
out, and on investigation many oscillating rods, of a length of 
O'OOlo — 0 006 mm., were to be found. On repeated knocking 
on the cover glass until almost the whole of the above particle 
had fallen to pieces, it was found swarming with oscillating 
rods, and in the broken edge of a large cell which had 
been fractured by the knocking a few rods were seen pro- 
truding into the fluid. 
