NOTES AND MEMORANDA. 
375 
animal by direct inoculation from a Rodent ; that the disease 
thus criven, though severe and possibly fatal, is not usually 
so, and that the m.odified attack of the disease confers a 
certain degree of protection from subsequent attacks com- 
municated in the same way ; so far, at any rate, as the 
experiments had been carried. 
(2) I showed also that the fungus which constitutes the 
essential contagium, when grown in successive generations 
in a cultivating fluid, was still capable of giving rise to the 
disease, being in one case fatal to a cow in the first genera- 
tion, in another fatal to a sheep in the fourth generation. 
My experiments also showed that an attack thus com- 
municated, causing severe symptoms, appeared to be equally 
protective against results from future inoculations with the 
disease given directly from the guinea-pig. 
I pointed out that, although I had applied as severe tests 
as were at my command, to ascertain the degree of protec- 
tion conferred, it was yet desirable to perform the more 
crucial test of direct contagion from another bovine animal ; 
and that, if the experiments were successful, the precise 
method of performing the protective inoculation, and the 
durability of the protection, would require further investiga- 
tion. Having regard, also, to the small number of animals 
as yet experimented on, it would be desirable to repeat similar 
trials on others. 
Keeping these points in view, I have continued the experi- 
ments, and have inoculated two other animals with similar 
results. Ill one case also, which had just recovered from the 
first attack at the time of my report, I have ascertained by 
further inoculations that protection had been conferred. 
Thus in all, I have added three more to the cases of success 
by this method. In one of these, the disease was given by 
the fourth generation of the cultivated fungus, and the 
symptoms were severe. 
8o far as concerns the crucial experiment, that of ex- 
])osure to direct contagion from another bovine animal, I 
liave not as yet had any opportunity of making it, and am 
still awaiting the occurrence of some outbreak, of which I 
liopc wo may receive early information. But I have just 
received some material from other animals which is known 
to be highly infectious, and am about to make experiments 
with it. 
Since my previous report, another very interesting and 
equally important point has become clear, which may I 
hope j>rove of great value in future. It is that, when the 
virus of the disease (the fungoid organism known as Raci7^W5 
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