40 Nebraska Agrici lti ral Exi\ Station Research Bi l. 10 
Table 7 (Continued) — Effect of freezing temperatures on ger- 
mination of corn differing in moisture content (1913 and 191b) 
Temperature range 16° to J2°F. 
Moisture 
range 
Moisture 
in grain 
Germination 
Ice 
observed 
in grain 
Embryos dark 
or light 
Normal 
grain 
Frozen 
grain 
Ratio 
of normal 
to frozen 
Per cent 
Per cent 
Per cent 
Per cent 
( 24.4 
100 
40 
100:40 
* 
Light 
23.9 
100 
100 
100:100 
* 
Light 
| 22.7 
100 
100 
100:100 
* 
Light 
| 22.1 
100 
100 
100:100 
* 
Light 
20 to 25. 
\ 21.5 
100 
100 
100:100 
* 
Light 
| 21.4 
100 
100 
100:100 
* 
Light 
| 21.0 
100 
100 
100:100 
* 
Light 
1 20.8 
100 
100 
100:100 
* 
Light 
[ 20.8 
100 
50 
100:50 
None 
* 
Average. . 
22.1 
o 
o 
87.8 
100:88 
f 19.8 
100 
100 
100:100 
* 
Light 
1 18.1 
100 
100 
100:100 
* 
Light 
17.8 
100 
100 
100:100 
* 
Light 
15 to 20 . . 
1 J 17.2 
100 
100 
100:100 
* 
Light 
17.0 
100 
100 
100:100 
* 
Light 
15.5 
100 
100 
100:100 
* 
Light 
( 15.3 
100 
100 
100:100 
* 
Light 
Average . 
17.2 
100 
100 
100:100 
*No observations made. 
noted thruout the range. The transition here from a fatal 
moisture content to one free from injury seems to have occurred 
between 30 and 25 per cent. Below this range, no definite in- 
jury appears to have resulted from the exposure as indicated by 
tin* percentage germination or by the color of the embryo. 
TEMPERATURE RANGE 8" TO 4° F. 
The data for the freezing tests in which a range of tem- 
perature from 8 to 4° F. was used are given in Table 8. In 
these tests, freezing and loss of germinative ability took place 
at a lower moisture content than in any of the previous tests. 
The transition here from injury to no injury seems to have 
occurred between 25 and It) per cent moisture content, and but 
little* loss of vitality appears to have resulted where the corn 
contained les> than 20 per cent of moisture. 
