NOTES ON SPOEOZOA TT. 
179 
nncleus has either not been quite parallel to the longer axis of 
the cell to start with, or else has become twisted round some- 
what by the entry of the parasite, the longer axis of the 
Karyolysns is more or less oblique to that of the host-cell 
nucleus, the one lying, as it were, across the other ; in these 
cases, fission of the host-cell nucleus into two or more portions 
ne.arly always results (figs. 29, 11, 30). An important point 
must be mentioned regarding the appearance which one of 
these nuclei, in its final condition of hypertrophy, may 
occasionally present on a Griemsa smear, since it affords, I 
consider, another example of how the over-staining tendency 
of this stain may mislead and cause erroneous interpretations. 
In a few cases a mass of staining substance is seen, fitting like 
a cap round one eud of the parasite, or there may be such a 
mass round both ends (figs. 16-18). These masses stain 
similarly to the nucleus of the host-cell, lying at one side of the 
parasite, and in fact may be distinctly connected with this and 
manifestly portions of it; it may happen, however, that such a 
mass appears almost or entirely separate from the nucleus, 
especially in flattened-ont parts of the smear. Nevertheless 
thei^ can be no doubt that these caps of staining substance 
represent also in such cases merely the wider, club-shaped end- 
portions of a crescentic host-cell nucleus, as above described, 
only here they are greatly overloaded with stain. These 
“ caps,” it is important to note, are distinctly on the outer side 
of the capsule enveloping the Hmmogregarine. 
As indicated above, the cytoplasm of the infected corpuscle 
becomes ultimately so colourless that it is quite impossible to 
di.scern it (c f. figs. 37, 38 from wet prepai’ations and fig. 17 
from a Giemsa smear) ; in tliese cases it cannot be said 
whether it is still present or not. 
The two forms of the parasite can now be considered in 
relation to the particular degree of alteration shown by the 
ho.st-cells respectively infected by them. As a rule, in 
corpiisles which ai-e in the earlier stages of altei’ation, with 
the nucleus still oval or only beginning to elongate, the 
parasites are of the first type described, with the nucleus 
