NOTES ON SPOEOZOxV II, 
183 
smaller and smaller^ being perhaps partially used up by the 
cytoplasm^ and ultimately its remains are seen at the surface of 
the body (figs. 34, 35). Often, however, no trace of this body 
is left (figs. 30, 38 and 39). On the other hand, occasionally 
this large karyosome seems to persist and to change its 
position with the nucleus (figs. 31, 37). In such cases it lies 
nearest to the end of the body, between this and the 
nucleus, having been pushed along as it were by the 
nucleus, instead of being left behind. Possibly the reason 
for this occasional persistence of the large karyosome 
as a separate element in close association with the nucleus, 
after the latter has changed its position, may be that 
the karyosome has not yet undergone the above-described 
division — a division which may be necessary in order to 
eliminate an unrequired portion of the karyosomatic material 
before the remainder is added to the nuclear substance. I 
have no evidence as to the further behaviour of the 
karyosome in these cases. 
1 can now summarise the general course of the early develop- 
ment in Karyolysus, so far as I was able to ascertain it. 
'I’he different types of form observed are phases of one parasite. 
A small individual, such as that of fig. 21, penetrates a red 
blood-eorpusele (fig. 19) and begins to grow. As the parasite 
grows, changes in the nuclear constitution and position take 
place. At about the same time a definite envelope or capsule 
is formed around the parasite, inside which the latter tends to 
acquire, by bending up, a characteristic U-shape, and ulti- 
mately becomes stout and bean-like. The presence of the 
llajinogregarine causes very great changes in the a])pearance 
of the host-cell. In pertrojdiy and pronounced alteration in the 
shape of the nucleus, sometimes its fission ; further, the 
cyto])lasm, or what remains of it, loses almost entirely its 
staining properties and becomes extremely difficult to see in 
the prepai’ations. 
Prom a comparison with lleichenow’s valuable and detailed 
account ( 27 ) of the develojnnent of 11 icm ogregarin a 
Stepan uvi of the tortoise, tho e can be little doubt that 
