216 
H. M. WOODCOCK. 
to suggest ; and I well remember that when I first saw such 
individuals in preparations, really well-fixed and stained,^ I 
felt no more doubt that Schaudinn’s view would prove to be 
correct than I felt about being at Rovigno. In my opinion, 
this remarkable resemblance was the foundation upon which 
Schaudinn built up his whole theory of the ontogenetic con- 
nection between the Trypanosomes and the intra-cellular 
parasites (Leucocy tozoon and Halteridium) of the little 
owl. To return to L. ziemanni, in the male gametocytes 
the great majority here also show no chromatin body in 
Giemsa-stained smears besides the large, oval, diffuse nucleus, 
tlie scattered granules of which stain faintly a pale red (fig. 7). 
Occasionally, however, two or three small bodies or grains, 
which may differ slightly in size and which stain red some- 
what more deeply than the nucleus, can be made out situated 
close together near the margin of the nucleus, forming as it 
were a clump almost in contact with it (fig. 9). These small 
structures are really only conspicuous in individuals which 
are if anything over-stained. Nevertheless the elements thus 
occasionally indicated in the nucleus of the male forms, 
stained by Giernsa, are found to be practically as constant in 
occurrence, in films stained by iron-haematoxylin, as is the 
single large body present in the female forms. 
' This remark is not made with any idea of self-praise ; it is by no 
means an easy matter to obtain Leiicocytozoon well fixed and 
stained, even according to the Romanowsky method, so as to show the 
nuclear structure pro^jerly, and also the different parts of the host-cell, 
in their true form and relation to the i^arasite. It is only necessary to 
glance at many of the figures of different species of Leucocy tozoon 
hitherto published to realise this. Either the parasites are hopelessly 
distorted and flattened out (cf. Dutton, Todd and Tobey’s figs. [2]), or 
the only sign of a nucleus is a space-like area in the middle of the 
cytoplasm (as in some of Mathis and Leger’s recent figures [3]) ; some 
of Wenyon’s figures, too, of L. neavei (8) are far from giving an 
accurate representation of the form and nuclear details. My figures in 
the present paper, as also those of L. fringillinarum in a previous 
memoir (9), show approximately the true nuclear appearance, as will be 
seen when the condition found in wet-fixed preparations stained by iron- 
hsematoxylin is discussed. 
