NOTES ON SPOUOZOA — 1\'. 
217 
The study of these g’anietoeytes of Leucocy tozoon in 
films stained with iron-haematoxylin is most instructive. 
Berliner, in his account of Flagellates (1), has also given 
figures of the intra-cellular pai-asites, Leucocytozoon and 
Halteridium, stained in this manner, with the idea of 
showing that they agree with the Binucleata in the possession 
of two nuclei (i.e. the occuri-euce of nuclear dimorphism); 
he does not, however, give any description of the details of 
unclear structure. As regards the female forms, the figures 
given by Berliner show, on the whole, the same appearance 
as that which I have found. 
Taking a general view, as it were, first, of the nuclear 
structure of the female gametocytes (figs. 11-17), this is seen 
to be, in many respects, of a similar type to that of the 
young schizonts of “Adelea.” For the most part the 
nucleus consists of a fairly large, central mass, which 
appears finely graunlar and stains to a moderate degree ; 
surrounding this the same clear, almost colourless zone can 
usually be made out, which is present in ‘^Adelea’'’ (cf. 
figs. 1-3). Berliner figures well-marked rays traversing this 
narrow zone ; now and again I am inclined to think I have 
caught a hint of the presence of one or two of these rays, 
but ill my preparations they are so faint and elusive that it 
is difficult to be certain. Standing out conspicuously by 
reason of the intensity with which it stains is the large 
chromatic body, which is so prominent in Giemsa-stained 
smears; this is always spherical and generally surrounded by 
a distinct halo, as is the karyosome in “Adelea.” It is 
usually in close association with the nucleus proper, though it 
may be distinctly separate from the latter, as in fig. 11, but I 
have never seen it so far removed as 1 have found it in 
Giemsa-stained preparations (cf. figs. 6 and 8). In two or 
three cases 1 have observed two such bodies, of unequal size, 
and neither so large as when there is only one, lying at 
opposite sides of the central mass (Kgs. 12, 17) ; the resem- 
blance of the nuclear condition in such cases to that seen in 
figs. 1 and 2 of “Adelea'’ and figs. 21, 2G, PI. 9, of 
