NOTES ON SPOROZOA— IV. 
219 
granule to tlie karyosotne (fig. 16). The larger granule may 
be present without there being any spindle or smaller granule 
(fig. 13). Very frequently close to this large granule is 
another one of about the same size and appearance ; but this 
latter appears to lie always outside the central mass of the 
nucleus, at the outer edge of the clear, surrouuding zone 
(figs. 14-16). 
Turning now to the male gametocytes, there is always a 
large, oval nuclear area. As in Gieinsa-stained smears, this 
is more usually very faintly stained (figs. 18-22) — remarkably 
so for a nucleus after irou-haeniatoxylin. It consists apparently 
of a loose reticulum with fine granules scattered throughout 
it. Here, also, this oval area is surrounded by a more or less 
distinct clear zone, but I have never, in this case, been able 
to make out any traces of rays crossing it, though Berliner 
(loc. cit.) just indicates a few in one of his figures pur- 
porting to be of male gametocytes. Berliner’s figures 
of male individuals, however, are much less satisfactory 
than those which he gives of female ones; and, in 
fact, I am very much inclined to doubt their lepre- 
senting male forms at all, for reasons which 1 will 
mention shortly. In the majority of cases the outer limit of 
the nucleus, external to the narrow, clear zone, is moie or 
less stiongly impregnated with chromatin, in the form of 
distinct granules, which stain deeply, and in optical section 
constitute a prominent chromatic ring, sharply delimiting 
the peripheiy of the nucleus (figs. 20-23). It is noteworthy 
that this well-marked peripheral zone of chromatic grannies 
in the male nucleus is apparently never to be observed in 
Giemsa-stained smears; it is not obvious iu <any of my pre- 
parations (for instance the individual of fig. 7 is on a smear 
made at the same time as the cover-slip preparation on 
which is the parasite of fig. 21), nor is it shown in any 
figures hitherto published. However, this zone is not always 
apparent, even iu iron-htematoxylin preparations; thus the 
individuals of figs. 18, 19 do not show it. Although, of 
course, the intensity of staining and the degree of extraction 
