220 
H. M. WOODCOCK. 
have a marked effect upon the prominence of this chromatic 
zone and the apparent size of the granules composing it, 
as equall^^ upon the appearance of the host-cell nucleus 
(cf. tigs. 22, 23), nevertheless I do not tliink tlie seeming 
absence of the zone in the instances mentioned is due, to 
any great extent, to the technique, i.e. to a less intense 
staining or to an excessive amount of extraction ; for one 
thing, both the host-cell nucleus may be more intensely 
stained, and the host-cell itself, i.e. its spindle-like pro- 
longations, more readily disceimible, in cases where the 
nucleus shows no chromatic zone than in cases where it does 
(cf, figs. 18, 19, and 20, 22). Again, while all the prepara- 
tions made from one infected owl may show the chromatic 
ring prominently, in those made from another bird this 
feature will be either not nearly so strongly marked, or else 
not discernible at all; this fact also points to a difference in 
this condition, in different cases or at different periods. 
I may emphasize the fact that I have never observed it in 
female gametocytes. 
Almost constantly associated with the male nucleus is a 
group of small, spherical, deeply staining elements. Very 
generally these are three in number; a larger, more external 
one and two smaller ones, of approximately equal size. The 
larger body is situated at the edge of the nucleus, or just 
outside the border or periphery (figs. 18, 19), and is often 
surrounded by a distinct halo. Both in position and appear- 
ance this element agrees closely with the large, conspicuous 
body associated with the nucleus in the female gametocytes, 
the only apparent difference being that it is never so large ; 
and I do not hesitate to suggest that it represents the same 
organella in the male forms, namely a karyosorne. Why this 
chromatic element should stain so much more easily and 
intensely with Giemsa in the case of the female individuals 
than it does in the male forms is, another instance of the 
peculiar and misleading vagaries of this stain. The two 
smaller elements 1 have mentioned, which apparently repre- 
sent a pair, are situated at about the limit of the central 
