NOTES ON SPOBOZOA — IV. 
221 
diffuse area of the nucleus, i. e. just internal to the narrow, 
clear zone (figs. 18, 19) ; (of course the disposition of these 
various organellse can only be correctly ascertained when 
they happen to lie in the plane of optical section). The two 
granules are sometimes connected by a short but distinct 
spindle (fig. 22) ; and iu one case (fig. 21) I have observed a 
spindle joining one of these granules to the larger body 
(karyosorne). 
It remains now to compare these granules occurring in the 
male nucleus with those described above in the female nucleus. 
It is highl}’ probable that the pair of granules in the male 
form corresponds to the two approximately equal-sized 
granules seen in the female gametocytes of figs. 14-16, near 
the periphery of the nucleus. There is a marked agreement, 
moreover, between the nuclear condition shown in figs. 21 
and 16, of male and female individuals respectively, where 
the large karyosome is still connected by a fibril with one of 
the two granules. A distinguisliing feature in all the cases 
I have observed is that iu the female nucleus the paired 
granules are radially arranged, while in the male they are 
tangentially arranged. The condition seen in the female 
individual of fig. 11, where the inner of the two granules has 
undergone a further unequal division, a still smaller granule 
remaining connected with it by a distinct centrodesmose, 
apparently represents a later phase which I have not seen in 
a male gametocyte. An important question is: Are these 
paired granules to be regarded as constituting kinetic elements 
(centrosomes) solely, or as representing small karyosomatic 
elements (i.e. containing alko chromatin) ? 'I’liat they contain 
a division-centre does not require to be emphasised, as this 
fact is clear from the various ceutrodesmoses I have described 
and figured in connection with them, in both male and 
female nuclei. In my opinion it may be regarded as certain 
that the very small peripheral granule seen, for instance, in 
fig. 1 1 is a centrosome (or centriole), still in connection with its 
fellow one; as, howeser, the body at the other end of the 
fibril is slightly larger, it may be, perhaps, that this latter 
