222 
H. M. WOODCOCK. 
element is really a very small daugliter-karyosome, possessing 
a certain amount of chromatin which encloses the true 
centriole (cf. the very small karyosoine and centriole in 
Hepatozoon can is, see p. 203). If this be so, the other 
g'ranule of the pair must also be interpreted as a small karvo- 
somatic element, and, of course, also the corresponding pair of 
granules in the male nucleus. 
To understand the exact significance of the somewhat 
complex system of divisions and resulting elements which I 
have described, a study of their behaviour during the further 
development, i.e. gamete-formation and fertilisation, would 
be necessary. From a consideration of figs. 14-16 it may 
})erhaps be suggested that the more external of the paired 
granules, situated usually just outside the clear nuclear zone, 
represents a further elimination of nnrequired nuclear material, 
j)ossibly a kind of maturation-process; but I have no indica- 
tion whether the same explanation holds good in the case of 
the male forms. Lastly, with regard to the lai’ge kai-yosome 
itself. Does this body contribute any of its store of chromatin 
to the general chromatic material dnriug the growth of the 
gametocyte, or is it entirely eliminated as unnecessary ? In 
this connection one point which I have noticed may be 
mentioned. The karyosoine is slightly but distinctly larger 
in a male nucleus wdiich does not show the chromatic zone 
than in one which possesses this feature (cf. figs. 18, 19, and 
20, 22). This may possibly indicate, in the latter case, some 
augmentation or replenishment of the chromatin of the general 
nuclear substance and a corresponding diminution of the 
amount held by the karyosorne. 
It will be clear, I think, that in regard to the essential 
features the nuclear constitution of both male and female 
gametocytes of Lencocy tozoon ziemanni shows a close 
agreement, and this notwithstanding the apparently pro- 
nounced differences shown when they are respectively stained 
by Giemsa. It is remarkable how constant in appearance, on 
the whole, the nuclear condition is found to be ; and this fact 
adds, of course, to the difficulty of interpreting the elements 
