NOTES ON SPOROZOA — IV, 
223 
observed While, however, the male and female nuclei of 
Lencocy tozoon are fundamentally similar in type, there is 
no possibility of mistaking the one for the other, even in films 
stained by iron-haematoxylin, on account of the constant diffe- 
rences in detail. As I have already mentioned, Berliner’s figs. 
50 and 53, PI. 29, which he regards as representing male 
gametocytes, do not agree at all with the characteristic appear- 
ance I have found and above described. The nucleus itself is 
figui-ed as round, instead of being, as it almost invariably is, a 
pronounced oval in shape ; and although it is somewhat larger 
than that of the female individuals which Berliner figures, it is 
nothin ike the size which the male nucleus usually is. More- 
over, the central area is stained more deeply, like that of the 
female forms, instead of being pale, even paler than the sur- 
rounding cytoplasm, as in the male forms; and lastly, there is 
no sign of the peripheral chromatic zone, d'he associated, 
intensely staining body is also very large, like the karyosome of 
the female gametocytes, and there is no indication of the small 
])aired elements close to it. In short, I feel almost certain that 
the individuals figured by Berliner as of male sex are leally 
also female forms (cf. his fig. 50 and my fig. 13, for instance). 
1 have dealt first with the nuclear structuie of Leuco- 
cy tozoon for two reasons: firstly, because in spite of its 
somewhat complex character it is not nearly so difficult to 
make out satisfactorily, on account of the large size of the 
parasites and the absence of pigment-grains, as is that of 
Halteridium, when fixed by a wet method and stained with 
iron-ha?rnatoxylin ; and secondly, because it is more readily 
comparable with the nuclear condition found in the young 
forms of “ Ad e 1 ea ” and Karyolysus. I have now to con- 
sider the nucleus of Halteridium, and will aarain besrin with 
the female gametocytes. Berliner (loc. cit.) in the explana- 
tion of his figures of this parasite says nothing at all about 
the sex ; so far as his figs. 58-GO, of fairly large or adult 
individuals, are concerned, these certainly represent female 
forms. No male forms are figured, just as I maintain is the 
case with his figures of Leucocy tozoon. The appearance 
