228 
H. M. WOODCOCK. 
individual figured, the conspicuous karyosoine no longer 
occupies a more or less central position within the nucleus, 
but has passed distinctly to the outside, and bears apparently 
the same relation to the general nuclear substance as does 
the karyosome of the female individuals of L. ziemanni 
drawn in figs. 13-16. The chief points of difference to be 
noted are that the nuclear substance is here so faintly stained 
that it appears more like a spherical space than a nucleus ; 
and secondly that I cannot (in this particular instance) make 
out any centrosomic granule. A similar condition is seen in 
figs. 33-35 of H. noctiue, but two of these parasites show a 
distinct centrosome which is appai'ently intra-nuclear, though 
it may, of course, be lying near the upper or lower surface. 
The nuclear condition in this case agrees very closely with 
that of the female gametocyte of L. ziemanni drawn in 
fig. 13. I have not observed a single instance, howevei-, 
where there are two granules in connection with the female 
nucleus of Halteridium, such as I have described as of 
frequent occurrence in Leucocy tozoon. It is most pro- 
bable, 1 think, that the centrosome^ seen in hgs. 33, 34 is the 
same element as that situated at the limit of the nucleus in 
figs. 24-26, but I have not found it connected by a fibril to 
the karyosome, where the latter has passed to the outside of 
the nucleus ; the fibril perhaps disappears when the karyosome 
changes its position. 
There can be no doubt, I think, that the smaller, intensely 
staining nuclear body in H. fringillfe (as seen when the 
parasites are stained by Giemsa), which I originally regarded 
as representing a kinetonuclear element, corresponds, not to 
the small peripheral centrosomic body seen in iron- 
ha 3 matoxylin preparations, when the nucleus has the conditiou 
shown in figs. 24-26, but to th e k ary osom e, when this 
has passed to the limit of, or outside the nucleus (as 
' In the case of the female nucleus also, I think it is preferable to 
regard this single granule as a centrosome only. I have not observed 
any secondary divisions or any further elimination (?) of small karyoso- 
matic portions, as in the female nucleus of Leucocytozoon. 
