NOTES ON SPOKOZOA IV. 
231 
as closely allied to tlie Coccidia ; it seems to me now that 
there is no longer any reason for supposing that they are 
derived from a binucleate form, such as a Haemoflagellate. It 
has been a great disappointment to me to find that the view 
so elaborately worked out by Schaudinn and apparently so 
firmly based on facts, which I in common with many 
other Protozoologists adopted enthusiastically, has had to be 
abandoned, step by step, until the entire edifice is seen to 
be without any true foundation whatever. From my own 
work I feel persuaded that the principal if not the only 
basis upon which Schaudinn built was that which I have 
above indicated, namely the remarkable resemblance between 
the nuclear condition of the female gametocytes of H alter i- 
dium and Leucocy tozoon, when stained by the Roman- 
owsky method, to that which a Trypanosome might be 
expected to show if in a resting phase. 1 greatly doubt, 
indeed, whether Schaudinn ever saw the nuclei of these 
gametocytes stained by iron-haematoxylin ; certainly no 
figures of individuals so stained are given in the recent 
published collection of his works (7). From the study by 
Minchin and myself (4) of this question, more especially from 
tlie standpoint of the Trypanosomes, and also from the 
present study of the cytology of the intra-cellular parasites, 
it must be admitted that no real evidence of any kind can be 
found to support Schaudinn’s view. 
Addendum. 
In view of the publication quite recently of a paper by 
Ih-owazek (5a), on the “ Geschlechtsdimorphismus der Try- 
})anosomen,” I feel obliged to add a few remarks to this note. 
Prowazek still maintains Schaudinn’s view that Leucocy - 
tozoon and Halteridium represent, in each case respec- 
tively, merely the sexual phases of a Trypanosome. He 
thinks that Mayer (‘Arch. I’rotistenk.,’ xxi, 1911), has suffi- 
ciently proved this idea in the case of Halteridium, and 
he himself endeavours to show that an actual connection 
