THE LEISHMANIA OF [TALIAN KALA-AZAlt. 
353 
On the Morphology of the Leishmania of Italian 
Kala-Azar. 
Third Communication^ : Cytological Researches on Leishmania 
in Cultures. 
By 
Dr. Arrig^o Viseiitiiii 
(From the Zoological Department of the Lister Institute of Preventive 
Medicine and the Institute of Comparative Anatomy of 
the University of Rome). 
With Plates 19 and 20. 
Researches on the minute structure of Protozoa, although 
initiated in coinpaimtively recent times, have already con- 
tributed to science a considerable number of facts of great 
interest, especially from the point of view of general cyto- 
logy. But while observ'ations conducted with the most 
delicate methods of cytological technique are feasible up to 
a certain point when dealing with Protozoa of a certain size, 
they meet with no slight difficulties when dealing with micro- 
organisms which, like the species of Leishmania, are of 
the smallest dimensions. hen, more than a year ago, I 
made successful cultures of Leishmania on the medium of 
Xovy, McXeal and Xicolle, by means of splenic juice 
obtained by puncture of the spleen of a young kala-azar 
patient of Bovalino Calabro, my teacher. Prof. Grassi, re- 
peatedly recommended me to undertake the present investi- 
gations with the special object of studying the little-known 
structure of the blepharoplast and the i-hizoplast, with 
* The author’s two previous communications on this subject were 
published in the ‘ Archiv f. Schiffs- und Tropenhygiene,’ xiv (1910), 
Beiheft 4, and ‘ Pathologica,’ iii (1912). 
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