THK LEISHMANIA OP ITALIAIST KALA-AZAR. 
355 
attached to the peripheiy or approximated to it. This ^'eosin- 
body ” (named by Leishmanu flagellar body ”) is found in 
the elong'ated forms at the anterior extremity of the para- 
site, always maintaining the same relations with the blephai’o- 
plast. It could be better described as an area coloured in 
pink ai-ound the blepharoplast and the rhizoplast, and it is 
supposed byFranchiui to have been found ultimately expelled 
from the parasite in the dried smears fixed in alcohol and 
stained with Giemsa’s stain. 
Wenyon also has occupied himself with the structure of 
L. tropica in cultures, and my researches, more complete in 
many points, will permit me, as will be seen in the sequel, to 
follow out still further the intimate morphological relations 
which exist between these two species, so closely allied, the 
ojie the specific agent of oriental sore, the other of kala-azar. 
I have had at my disposition three sources of cultures. 
Besides that obtained by myself and mentioned above, I have 
7-eceived other cultures from the Pasteur Institute of Paris, 
which originated from Nicolle in Tunis, and others veiy 
recently from the Clinica Pediatrica of Palermo. To Messrs. 
IMesnil and Jemma, who so kindly sent me them, I return iny 
best thanks. 
The results obtained from these different cultui-es agree 
perfectly, as was to have been expected, so that in the descrip- 
tions of my observations I refer to the one or the other 
indifferently. 
When preparations are to be made of flagellates in a 
culture on blood-agar, it is a most important precept that 
the smeai’s should be very thin, otherwise the flagellates for 
the most part do not become differentiated, and Protozoa 
also stain in an incomplete manner; moreover the examina- 
tion is hindered by the presence of hgemoglobin particularly, 
which takes an intense colour, and in which the Protozoa are 
imbedded. 
The smears on a cover-glass or slide were made with a 
small drop of the culture by means of another cover-glass or 
slide, smearing the material iu a backward direction. 
