360 
ARBIGO VISENTINI. 
From the karvosorne very delicate filaments (liniu) can 
sometimes be seen to arise, connecting the karyosorne to the 
nuclear membrane in a radiate manner, and showing here and 
there very minute granules connected to one another iu their 
turn by transverse threads. On the inner surface of the 
nuclear membrane, granules and dots (Chromatinkomplex of 
Schaudinn) are observed united to the karyosorne by delicate 
filaments. But in general the linin-franiework and the 
chromatin of the nuclear sap-zone are very feebly developed 
in the principal nucleus of Leishmania and the granules 
extremely minute. 
With the method of Giemsa, after wet fixation (fig. 3) it is 
also possible to demonstrate the minute structure of the 
nucleus in all its constituent parts ; on the other hand, in 
dried smears Giemsa’s stain usually colours the sap-zone also, 
so that the nucleus appears as a rounded homogeneous body, 
in the interior of which some granules of chromatin more 
intensely stained can be made out with difficulty. 
In the smears stained Avith iron-htematoxylin there are 
sometimes found in the nuclear sap-zone of the principal 
nucleus one or even two granules intensely stained, larger 
than those Avhich are met with normally along the threads of 
the linin-netAvork and easily distinguished fi-om them. Their 
number, one or tAvo, is especially characteristic, and in fig. 42 
I have represented a nucleus in Avhich the tAvo granules are 
connected by a very delicate filament, not to be confounded, 
it appears to me, Avith a filament of linin. We are dealing 
here, perhaps, AVith the centriole or extra-karyosomic centro- 
some dividing into two for the first time as a prelude to the 
division of the Avhole nucleus ; or is this a phenomenon of 
chromatinic reduction, like others of Avhich I shall give an 
account in the sequel ? 
The kinetonucleus, situated betAveen the trophonucleus and 
the anterior extremity of the body, but nearer to the former 
than to the latter, appears composed mainly of a fusiform or 
oval body situated Avith its greater diameter in a transverse 
direction, compact and intensely stained, representing the 
