THE TRANSMISSION OP LEISHMANIOSIS BY CULTURES. 373 
The Transmission of Leishmaniosis by means of 
Cultures, and the Mechanism of the Natural 
Immunity in Rats and Guinea-pigs. 
By 
Dr. Arri^o Viseiitiiii. 
With Plate 21. 
Attempts to transmit to animals the infection of Leisli- 
mania by means of cultural forms have not always had 
constant results up to the present. 
Nicolle (1909) first inoculated dogs and monkeys (animals 
notoriously susceptible to leishmaniosis) with 1 c.c. of a 
culture, but without result ; afterwards, however, in col- 
laboration with Manceaux, he obtained positive results by 
inoculating Macacus cynomolgus and M. sinicus, repro- 
ducing the infection with the clinical picture of infantile 
leishmaniosis. 
Novy (1908), by means of cultures obtained from Nicolle, 
had already succeeded in infecting dogs and afterwards other 
laboratory animals (?) by inoculating considerable quantities 
of the parasites, in the case of one dog about 270 cullm’es in 
fifty injections. Subsequently, however, Novy (1909) estab- 
lished the fact that a single injection of a suspension obtained 
from a score of culture- tubes is sufficient to produce leish- 
maniosis in dogs. 
The rabbit has so far been proved to be susceptible only to 
corneal injection and to the forms occurring in the hteinato- 
poietic organs. Volpino, by scarification of the cornea with 
material obtained from a dog infected with experimental 
leishmaniosis, produced a keratitis similar, up to a certain 
