:J82 
DR. ARRIGU YISExVTlXl. 
gulfed by the leucocytes, the mononuclears exclusively, and 
undergo gradual and progressive alteiations, ending in their 
complete destruction, so that by the end of an hour or an 
hour and a half after the injection of 2 c.c. of a culture in 
full development, free flagellates are no longer to be found 
in the serum, and after two or three hours even the last 
vestiges of them are almost destroyed in the protoplasm of 
the phagocytes. 
The Protozoa do not pass beyond the barrier of the peri- 
toneum and do not find their way in this manner into the 
blood or the internal organs. 
These data of observation receive full confirmation in the 
results of the cultures. It is possible, in fact, to obtain sub- 
cultures from the peritoneal fluid up to about one hour after 
the injection of 2 c.c. of liquid of condensation very rich 
in Leishmanias, but not beyond this period, nor are sub- 
cultures obtained from the blood or organs of the animals in 
experiment. 
I desire to express my gratitude to Dr. Martin for having 
received me with so much kindness and hospitality at the 
bister Institute of Preventive Medicine, London, of which he 
is the Director, and for having provided me liberally with 
the means of research ; I also thank Dr. Bayon warmly for 
assisting me in every way during my stay in this Institute. 
Lister Institute of Preventive Medicine, 
London, 
May 2.5tli, 1912. 
Literature. 
1. Basile, C., La Cava, F., and Visentini, A. (1911). — “Soprauncaso 
di leptomeningite da Leishinania,” ‘Bend. Acc. Lincei’ (5), 
XX, p. 69. 
2. Delanoc, P. (1911). — “ L'ininnmite natnrelle de la souris a I’egard 
des cultures de Kala-Azav et de Bouton d’Orient tunisien,” 
‘ C.R. Soc. Biol., Paris,’ Ixx, p. 387. 
3 . (1912). — “ L’importance de la pliagocytose dans rimmunite 
de la souris a I'egard de quelques flagelles,” ‘ Ann. Inst. Pastern-,’ 
xxvi, p. 172. 
