454 
J. GRAY. 
a particular chromosome ; the smaller vesicles would represent 
parts of such complexes — the remainder of which have gone 
to form chromosomes in the usual way. 
The staining reactions of these vesicles are peculiar. They 
stain blue-black in Heidenhain’s haematoxylinj dark blue in 
Ehrlich’s hmmatoxylin, and maroon in Ehrlich-Biondi-Heiden- 
hain. Whether they are to be regarded as chromatic or 
nuclear in nature is more fully discussed in another place. 
Series C. — Eggs of E. esculentus treated with 50 c.c. 
sea- water + 7 c.c. 2| M. NaCl solution. 
The effect of a solution of this strength upon the chromatin 
varies a great deal for individual eggs: a more or less com- 
plete series can be found between eggs with normal mitotic 
tigureSj and eggs in which the chromatic complex is entirely 
disorganised. 
Eig. 18 shows an advanced anaphase, in which the individual 
chromosomes are clearly visible, each possessing its charac- 
teristic shape ; the fig-ure is, however, somewhat irregular, and 
the chromosomes, instead of being arranged in two definite 
plates are considerably scattered on the spindle towards the 
two poles. Two chromosomes are seen lying entirely off the 
mantle fibres. 
Fig. 19 shows another anaphase Avhich is slightly more 
irregular, but in which most of the chromosomes have 
retained their individuality. They have moved towards the 
poles in an irregular manner, the lower group especially 
being very irregular. In both groups it is seen that a few 
chromosomes have lost their normal shape, and have swollen 
up to form rounded or irregular masses of chromatin; as 
in fig. 18, a few chromosomes have separated from the main 
spindle. Fig. 62 shows the two ends of an anaphase in 
face view; nearly all the chromosomes are swollen, and in 
the right hand group is a large irregular mass of chromatin. 
Fig. 20 shows a prophase condition which apparently pre- 
ceded such an anaphase as is shown in Fig. 19; the 
aggregation of some of the chromosomes into clumps i& 
very characteristic. 
A 
