460 
.T. GRAY. 
cliromosomes. There is no doubt that this is the case. Fig. 
64 shows an anaphase in one group of whicli there are 34 
chromosomes, and in the other 35 — while between the two are 
two large vesicles, two chromosomes, and a small dot (the 
latter being larger than a normal chromosome and yet not 
definitely vesicular). No\v if each of these large vesicles re- 
presents one of the 38 chromosomes of the original nucleus — 
then the total number of potential chromosomes would be 
34 -f 35 + 7 = 76, which is the full number for an anaphase 
of a normal egg. Fig. 65 shows an aggregate of 76 in a 
similar way. 
From figs. 36-45 it will be seen that the behaviour of these 
vesicles is exactly similar to those described for “ hypertonic ” 
E. esculentus eggs, and for the hybrid E. esculeutus S 
X E. acutus ?. Fig. 40 shows the omission of two 
vesicles, and the inclusion of one vesicle among tlie chromo- 
somes at each end of the spindle. A tripolar spindle with 
about seven vesicles is shown in fig. 48. 
Figs. 49 and 50 show two aberrant nuclei comparable to 
G. Hertwig’s figs. 19-23 and 26, and Konopacki’s fig. 29. 
If it could be shown that the vesicles in these eggs were 
always formed from the same individual chromosomes, there 
would be an interesting’ proof of the physiological individuality 
of these bodies. Unfortunately, however, treatment with a 
hypertonic solution of this strength often causes the normal 
shape of most of the chromosomes to be lost, so that the missing 
members of the complex cannot be identified by this means.^ 
All that can be said, is that a certain number of chromosomes 
become converted into vesicles, from what elements in the male 
or female chromatin they are derived it is impossible to say. 
It is just possible that those vesicles which are found with- 
in tlie nuclear membrane are really nucleoli, while those which 
are formed directly from the chromosomes must be regarded 
' In the normal complex there can be identified two rod-like chromo- 
somes which are distinctly longer than the rest ; in these “ hypertonic ” 
eggs only one such chromosome can be recognised, but, as shown in the 
text, this evidence cannot be regarded as very satisfactory. 
