468 
J. OKAY. 
der gesainuite Kern rot farbende Koruer aiifgetreten, die in 
ihrem Iniieni eine Art Vacuole aufweisen. . . . Icli 
glaube diese Kornchen als Nucleoleu denten za diirfeu/’ 
Tliere can be little doubt that these “ Kornchen ” are to be 
identified with the “ vesicles^’ found within the nuclear mem- 
brane of the eggs of E. esculentus and E. acntus, which 
have been treated with hypertonic sea-water. 
Now, in series B ol the eggs of E. acutus, it has been 
shown that the formation of the vesicles is attended by a 
reduction in the number of the chromosomes, and yet these 
vesicles do not show a chromatic reaction with Ehrlich- 
Biondi-Heidenhain’s stain. This is, however, not a con- 
clusive proof that the vesicles are not derived from the 
chromosomes, because the normal chromosome vesicles, which 
are formed directly from the chromosomes in the telophase of 
Echinoid eggs, are also stained pink by this reagent. Hence 
it seems justifiable to assume that the formation of the 
vesicles and the elimination of them from the rest of the 
chromatin of the nucleus is equivalent to the elimination of 
the chromosomes themselves, and that these vesicles are 
not necessarily of nucleolar origin — a conclusion which 
receives very strong support from the mode of formation 
of these vesicles themselves. Hertwig’s fig. 24 makes it 
appear as though in some cases the nucleus divides into two 
without the disappearance of the nuclear membrane, by a 
process of “ainitosis,” such as Kouopacki described (see 
Konopacki’s figs. 31 and 33). In other cases (as when the 
sperm received lower intensities of radium treatment) the 
mitotic figures are more normal, a typical spindle and asters 
being formed. The chromatin in these cases, however, is 
seen to be quite abnormal, for, while some of the chromo- 
somes are i-ecognisable as rod-like bodies, a considerable 
number are represented by irregular masses of chromatin 
scittered on the spindles (which are often multipolar, as lu 
the eggs of E. esculentus here described) (cf. his figs. 28- 
32). Most of this chi’omatin scheint teils in Form der 
kleinen Kornchen vorhanden zu sein, teils hat es sich auch 
