540 CEESSWELIi SHEAUEE AND DOEOTHY JOEDAX LLOYJ). 
obtained good results with tlie proportion used by Delage, 
namely, 15 c.c. of sea-water to 35 c.c. of sugar. Table IX 
gives a series of experimeuts varying these proportions. 
Table IX. 
Lot. I. Lot II. 
55°/o eggs showing . 50° eggs showing 
attempt at clea- iri'egular clea- 
vage. A few vage. 
blastulse. 
15°/o blastulsB. . Cleavage fairly 
More cytolysis. regular. 25 °/q 
blastulae. 
50° /o good bias- . Cleavage less regu- 
tulee lar than in (2). 
15°/o blastulae. 
. Very few attempts 
at cleavage. 
The experiments given in Table X were made to vary the 
proportion of ammonia to tannic acid. It indicates that 
Delage’s method of adding '1 c.c. more of ammonia than of 
tannic acid gives the best results. A higher proportion of 
ammonia is accompanied by increased cytolysis. 
Table X. 
(a) 15 c.c. sea-water -)- 35 c.c. 113 M sugar + 1'4 c.c. M;60 tannic acid 
for 6 mins. 
(b) (1) 1'4 c.c. N/10 ammonia added for 1 hr. . 70 °/q eggs showing 
cleavage, 30 °/q blas- 
tulre. 
(2) 1'5 c.c. ditto 35 °/o blastulae. 
(3j T7 c.c. ditto ...... Some superficial cyto- 
lysis, cleavage more 
irregular than (1) 
and (2). A few blas- 
tulae. 
(4) I’O c.c. ditto Large amount of cyto- 
lysis. Cleavage very 
irregular. 
(1) 25 c.c. sea-water + 25 c.c. . 
1T3 M sugar solution, 
1"4 c.c. M/60 tannic acid 
for 6 min., 1'5 c.c. N/10 
ammonia for 60 min. 
(2) 15 c.c. sea-water -|- 35 c.c. . 
ditto 
(3) 10 c.c. sea- water + 40 c.c. . 
ditto 
(4) 5 c.c. sea- water -|- 45 c.c. . 
