FUETHEH NOTES ON A TRYPANOSOME. 
125 
clear area. At 6.15 the two nuclei were once more quite 
clearly defined but the clear area joining them remained 
visible till about 9 o’clock after which it was no longer to be 
detected. This clear band is the remains of the division 
spindle : it is a very characteristic feature in the stained 
specimens. During the division of the nucleus the body had 
gradually become flattened in an antero-posterior direction 
and correspondingly widened laterally. Grooves also began 
to appear in the antero-posterior direction. It is to be noted 
that these arose both at the anterior and the posterior end — 
the anterior end being the broad end at which the nucleus 
lies when the animal is in the pear-shaped condition and at 
which the flagellum is later developed. 
These gTooves altered a good deal in appearance during the 
next few hours and towards 4 a.m. had deepened till the 
animal presented the picture of two pears stuck together in 
the middle, with however, the two broad ends and the two 
pointed ends free. This is a point of some slight importance. 
When in the trypaniforiu state division of the protoplasm of 
this parasite usually begins from the anterior end and pro- 
ceeds to the posterior. This is likewise the rule in the crithi- 
dial state. This question of the grooves arising at both the 
ends of the parasite is not in itself deserving of much remark 
but it explains some curious stages where division of the 
protoplasm goes from the posterior to the anterior end, to be 
described in a later part of the paper. 
This individual was watched for another two hours and one 
of the two daughter-individuals developed a stiff and very 
short flagellum which, however, showed no signs of move- 
ment. The other individual appeared to have a little projec- 
tion suggesting a flagellar rudiment. At 0.30 a.m. the animal 
was finally abandoned, although the complete separation of 
the pi'otoplasm had not yet occurred. 
The flagellum seems to appear for the first time somewhere 
between the second and third day after feeding. It is a very 
characteristic feature that it generally arises at a division 
stage. The flagellum is at first a stiff and relatively thick 
