FURTHER NOTES ON A TRYPANOSOME. 
127 
many rounded forms, some dividing and some developing tlie 
flagellum, were likewise present. Some of the trypanifonn 
individuals were already dividing. 
Conjugation was very carefully searched for as it seemed 
pi’obahle that it might occur at this stage of the life cycle, 
but no signs of such a process were detected. Two individuals 
were found joined by their posterior ends, one slightly broader 
than the other. They were watched continuously from 6 p.m. 
till 3.15 a.m., and the protoplasmic junction between the two 
was seen to become much more slender and pulled out, 
showing that the individuals were dividing. 
Text-fig. 4. 
Text-fig. 5. 
Text-fio. 4. — Rounded off Trypanosome. 
Text-fio. o. — Division of resting Trypanosome. 
Drawings made from live specimens in a sealed slide of skate’s 
blood. 
An interesting corroboration of the stages above described 
was obtained from blood drawn from a skate and sealed up 
between a coverslip and slide (text-figs. 2 — 5). A Try- 
panosome was continuously watched from 2.45 p.m. when the 
slide was made. At 4.30 the animal had come to rest. The 
flagellum which, when it breaks free from the membrane, is 
seen to be of a relatively immense length was tangled up 
round the animal. The slide was watched for some hours 
longer, but as the Trypanosome was no longer motile it was 
left. Xext morning it was found to have divided into two. 
The behaviour of the Trypanosomes on a sealed slide is 
interesting — a number do not alter at all, others very soon 
after the slide is made begin to react to the altered condi- 
